Fig. 5:

miR-21-5p inhibits the melanogenesis of melanocytes via targeting SATB1. PIG1 cells were transfected with miR-21-5p mimic and SATB1 overexpression plasmid for 24 h. (A) The transfection efficiency of SATB1 overexpression plasmid in PIG1 cells was confirmed by RT-qPCR assay. (B) Western blot assay showed the transfection efficiency of SATB1 overexpression plasmid in PIG1 cells. (C) Western blot assay showed SATB1 protein expression in PIG1 cells. (D) Melanin content in melanocytes was detected by a microplate reader. (E) Tyrosinase activity in melanocytes was detected by a microplate reader. (F) The mRNA levels of MITF, tyrosinase, TRP1 and TRP2 were assessed by RT-qPCR assay. (G) The protein levels of MITF, tyrosinase, TRP1 and TRP2 were assessed by western blot assay. *P < 0.05, **P < 0.01, ***P < 0.001