FIG. 2.

Chitin-treated mouse spleen cells decreased MPB-59-stimulated IL-4, IL-5, and IL-10 production but increased MPB-59-stimulated IFN-γ production. Spleen cells were isolated from the WT mouse groups receiving the indicated treatment and stimulated in vitro with MPB-59 at 0 (medium), 10, 20, and 50 μg/ml for 4 days. The cytokine levels in the culture supernatants were measured by ELISA, as described in Materials and Methods. Values are means plus standard deviation from triplicate cultures. The data shown are representative of two independent experiments. ∗, ∗∗, and ∗∗∗, P < 0.05, P < 0.01, and P < 0.005 compared to the MPB-59-immunized group.