FIG. 3.

Chitin-treated mouse spleen cells decreased MPB-59-stimulated IL-4 and IL-5 production but increased MPB-59-stimulated IFN-γ production in IL-10-KO mice. IL-10-KO mice were immunized as described in Materials and Methods. Recall responses of spleen cell cultures were assayed as described in the Fig. 1 legend. Values are means plus standard deviations from triplicate cultures. The data shown are representative of two independent experiments. ∗, ∗∗, and ∗∗∗, P < 0.05, P < 0.01, and P < 0.005 compared to the MPB-59-immunized group.