FIG. 5.

Development of MPB-59-induced footpad DTH in WT and IL-10-KO mice coimmunized with MPB-59 and chitin. WT and IL-10-KO mice were immunized with MPB-59, MPB-59–chitin, chitin, and saline as described in Materials and Methods. Seven days after the final immunization, mice received 50 μg of MPB-59 solution in the right footpad and 50 μl of saline in the left footpad (control). After 48 h, right footpad thickness minus left footpad thickness in each group of mice was calculated. Values are means plus standard deviations; n = 6. ∗ and ∗∗, P < 0.05 and P < 0.01 compared to the MPB-59-immunized group.