Table 2.

Primer sequences of target genes used for RT-qPCR.

Target genes	Primer sequences	PCR products (bp)	Reference/Gene bank accession	Annealing temperature(°C)	Amplification efficiency (%)
Claudin-1	5′-CACCCTTGGCATGAAGTGTA-3′ 3′-AGCCAATGAAGAGAGCCTGA-5′	216	XM_005675123.3	63.1	97.91
Occludin	5′-GTTCGACCAATGCTCTCTCAG-3′ 3′-CAGCTCCCATTAAGGTTCCA-5′	200	XM_018065677.1	62.7	98.83
ZO-1	5′-CGACCAGATCCTCAGGGTAA-3′ 3′-AATCACCCACATCGGATTCT-5′	163	XM_018066114.1	62.2	97.08
FASN	5′-GTGTGGTACAGCCCCTCAAG-3′ 3′-ACGCACCTGAATGACCACTT-5′	110	XM_015098375.1	65.2	96.87
mTOR	5′-TGGTTTGGTGAAACCAGAGG-3′ 3′-AGAGCTGAACTTTCTGACCAT-5′	294	NM_001285748.1	62.3	96.19
AKT	5′-AGGCCAAGTCCTTGCTCTC-3′ 3′-ATCATCTGGGCTGTGAACTC-5′	217	NM_001382431.1	62.7	99.62
4EBP1	5′-GGAACTCACCTGTGACCAAA-3′ 3′-ATGGCTGGTGCTTTAAATGTC-5′	182	NM_004095.4	62.3	99.72
P70S6K	5′-CTCTCAGTGAAAGTGCCAAC-3′ 3′-TTGGAAGCGGTGCTGAA-5′	297	NM_001285641.1	61.7	97.93
β-actin	5′-GTGACGTTGACATCCGTAAAGA-3′ 3′-GCCGGACTCATCGTACTCC-5′	245	XM_018039831.1	63.5	99.55
PI3K	5′-TCCAGCACATGAACGTAAACAG-3′ 3′-GAAACTCGGTGACTCACACAC-5′	143	XM_028674370.1	63.9	96.65