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. 2022 Oct 27;8(2):e10436. doi: 10.1002/btm2.10436

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© 2022 The Authors. Bioengineering & Translational Medicine published by Wiley Periodicals LLC on behalf of American Institute of Chemical Engineers.

This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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(a) Illustration of the MILF strip assay for neutralizing antibody test to SARS‐CoV‐2 and evaluation of the analytical performance of the SARS‐CoV‐2 MILF strip. (b) Binding interference between ACE2 and SARS‐CoV‐2 RBD was tested using hCypA. The images and signals of strips exposed to different hCypA concentrations were analyzed by ImageJ software. (c) The calibration graph as control line (C line) and test line (T line, ACE2 zone) ratio of hCypA concentration. (d) MILF strip assay that highlights the neutralizing ability of hCypA was confirmed at the T line using AuNP‐variant RBDs. MILF strip assay test results of the hCypA with the AuNP‐variants RBDs (e), and the calibration graph as the T/C ratio (f).