TABLE 3.
SPR binding affinity values of ACE2–RBD variants, hCypA–RBD variants, and ACE2 with hCypA–variants RBD complex
| Ligand | Receptor (SARS‐CoV‐2 RBD) | Ka (1/M*s) | Kd (1/s) | K D (M) |
|---|---|---|---|---|
| ACE2 | Alpha | 3.08 × 104 | 2.14 × 10−4 | 6.97 × 10−9 |
| Beta | 1.02 × 106 | 2.887 × 10−2 | 2.83 × 10−8 | |
| Gamma | 2.88 × 105 | 1.002 × 10−3 | 3.49 × 10−9 | |
| Delta | 2.57 × 105 | 8.21 × 10−4 | 3.20 × 10−9 | |
| Epsilon | 2.55 × 106 | 2.153 × 10−1 | 8.46 × 10−8 | |
| Kappa | 9.76 × 104 | 3.682 × 10−3 | 3.77 × 10−8 | |
| Lambda | 2.50 × 105 | 1.753 × 10−3 | 7.01 × 10−9 | |
| Omicron | 2.58 × 104 | 2.293 × 10−3 | 8.90 × 10−8 | |
| Deltacron | 6.59 × 104 | 4.519 × 10−4 | 6.85 × 10−9 | |
| hCypA | Alpha | 2.37 × 105 | 1.029 × 10−2 | 4.34 × 10−8 |
| Beta | 4.04 × 105 | 3.719 × 10−2 | 9.21 × 10−8 | |
| Gamma | 2.25 × 104 | 8.305 × 10−3 | 3.69 × 10−7 | |
| Delta | 6.60 × 104 | 3.97 × 10−2 | 6.01 × 10−7 | |
| Epsilon | 3.17 × 104 | 2.405 × 10−3 | 7.59 × 10−8 | |
| Kappa | 1.14 × 105 | 9.444 × 10−3 | 8.28 × 10−8 | |
| Lambda | 5.38 × 104 | 3.815 × 10−3 | 7.09 × 10−8 | |
| Omicron | 6.26 × 104 | 1.643 × 10−3 | 2.64 × 10−8 | |
| Deltacron | 5.77 × 104 | 1.072 × 10−3 | 1.86 × 10−8 | |
| ACE2 | hCypA + Alpha complex | 2.88 × 102 | 3.788 × 10−3 | 1.32 × 10−5 |
| hCypA + Beta complex | 7.63 × 102 | 5.889 × 10−2 | 7.72 × 10−5 | |
| hCypA + Gamma complex | 6.51 × 102 | 4.879 × 10−2 | 7.49 × 10−5 | |
| hCypA + Delta complex | 1.39 × 104 | 3.10 × 10−4 | 2.23 × 10−8 | |
| hCypA + Epsilon complex | 2.48 × 100 | 1.92 × 10−4 | 7.74 × 10−5 | |
| hCypA + Kappa complex | 1.23 × 100 | 2.30 × 10−4 | 1.87 × 10−4 | |
| hCypA + Lambda complex | 1.76 × 100 | 3.55 × 10−4 | 2.02 × 10−4 | |
| hCypA + Omicron complex | 1.44 × 100 | 2.98 × 10−4 | 2.08 × 10−4 | |
| hCypA + Deltacron complex | 1.69 × 100 | 2.46 × 10−4 | 1.46 × 10−4 |