TABLE 2.
Iron transport systems in E. coli CFT073 and its isogenic iron uptake mutants
| Straina | FeSO4 utilizationb | Hemin utilizationb | Aerobactin
|
Enterobactin
|
||
|---|---|---|---|---|---|---|
| Synthesis | Transportb | Synthesis | Transportb | |||
| CFT073 | + | + | + | + | + | + |
| CFT073-TB | + | − | + | − | + | − |
| CFT073-TB(pYUK1) | + | + | + | + | + | + |
| CFT073-LH | + | −c | + | + | + | + |
| CFT073-ENT | + | + | + | + | − | + |
| CFT073-IUC | + | + | − | + | + | + |
| CFT073-ENT/IUC | + | + | − | + | − | + |
| CFT073-IUT | + | + | + | − | + | + |
Abbreviations: -TB, tonB; -LH, chuA; -ENT, entF; -IUC, iucB; -ENT/IUC, entF iucB; -IUT, iutA; pYUK1, TonB+ plasmid.
Bioassays were performed by seeding the strain in L-EDDA agar and spotting the indicated compound on the surface of the plate. + indicates a zone of growth around the iron compound. − indicates no stimulation of growth. Synthesis of siderophores was determined by chemical assays and bioassays.
A small amount of background growth was observed on occasion.