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. 2023 Jan 11;13:1076298. doi: 10.3389/fpls.2022.1076298

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Copyright © 2023 Anders, Wilson, Sorieul, Nikolovski and Dupree

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The CTS of GT43s, their potential interactions and impact of interactions on Golgi localization. (A). Sequence logos showing conserved motifs in the predicted transmembrane helices (TMH) of orthologs of IRX9/IRX9L (top) and IRX14/IRX14L (bottom). (B) Close-ups of AlphaFold-Multimer models of the transmembrane dimers for an IRX9 homodimer (top), an IRX9–IRX14 heterodimer (middle) and an IRX14 homodimer (bottom). Residues of the GAS_right motif and conserved cysteines are highlighted in magenta or dark blue, respectively. (C) Subcellular localization of Arabidopsis IRX9-GFP, transiently expressed in tobacco leaves. Top panel (left to right), IRX9-GFP (green), ManI-mCherry from soy (Golgi marker, red), merge of the two former, and merge including differential interference contrast (DIC) image. Bottom panel shows the same localization analysis in presence of co-expressed, untagged IRX10 and IRX14. Note the change of localization of IRX9-GFP in presence of IRX10 and IRX14.