Figure 1.

Testicular tissue composition in adult transwomen and control groups. (A) PAS stainings representing the four categories evaluated for the point counting analysis: tubule with germ cells (wGC), tubule without germ cells (without GC), tubular shadow (Ts) and interstitium (Int). The relative proportions of the categories were determined for pre-/pubertal controls (n  = 5, B), adult controls (n  = 5, C) and transwomen (n  = 25, D). Scale bar: 50 µm. Graphs show mean ± s.d. Data were analyzed by non-parametric Kruskal–Wallis test and significant differences between the categories are marked with ^**P < 0.01 and ^***P < 0.001.