Figure 4.

Relacorilant exhibits GR antagonism in peripheral tissues but not in the CNS. After 3 consecutive days of treatment with either vehicle, 60 mg/kg mifepristone or 60 mg/kg relacorilant, male mice were injected with 5 mg/kg DEX 1 h after the last treatment, and tissues were harvested 6 h later. The effect of relacorilant and mifepristone treatment on DEX-induced GR target gene expression (Fkbp5, Gilz, Mt2a) in mouse (A-C) liver, (D-F) adrenal gland, (G-I) pituitary and (J-L) hippocampus. All data are expressed as mean ± s.e.m. Statistical significance was calculated using GraphPad Prism 8 using a one-way ANOVA. DEX, dexamethasone; Mif., mifepristone; Rela., relacorilant; Fkbp5, FK506-binding protein 5; Gilz, glucocorticoid-induced leucine zipper protein; Mt2a, metallothionein 2A; 18S, 18S rRNA; 36B4, ribosomal phosphoprotein P0; B2M, beta-2-microglobulin.