Figure 5.

Relacorilant exhibits GR antagonism in mouse pituitary AtT20 cells. The effect of different doses of relacorilant or mifepristone on the effects of 5 nM DEX on (A) Fkbp5, (B) Gilz, (C) Nr3c1, (D) Pomc expression, and (E) ACTH release. All data are expressed as mean ± s.e.m. The IC50 values were determined using GraphPad Prism 8 non-linear fitting. The relative expression of genes was normalized using the geometric mean of the expression of three reference genes (RG) Hprt, GusB and Gapdh. DEX, dexamethasone; Mif., mifepristone; Rela., relacorilant; Fkbp5, FK506-binding protein 5; Gilz, glucocorticoid-induced leucine zipper protein; Nr3c1, nuclear receptor subfamily 3 group C member 1; Pomc, proopiomelanocortin; ACTH, adrenocorticotropic hormone; Hprt, hypoxanthine phosphoribosyltransferase 1; GusB, glucuronidase beta; Gapdh, glyceraldehyde-3-phosphate dehydrogenase.