Figure 6.

Relacorilant induces a unique GR interactome. (A) Interaction score of GR with 154 short leucine-rich binding motifs of 64 known nuclear receptor coregulators as measured by the modulation index (MI) in the presence of 1 µM cortisol, mifepristone or relacorilant. (B) Fold change-fold change plot of the interaction score of GR with nuclear receptor coregulator motifs as measured by the modulation index (MI) in the presence of 1 µM relacorilant compared to 1 µM mifepristone. (C) Interaction score of GR with a subset of nuclear receptor coregulator motifs as measured by relative binding in the presence of 1 µM cortisol, mifepristone or relacorilant. The relative interaction is expressed as mean ± s.e.m. and statistical significance was calculated using a one-way ANOVA in GraphPad Prism 8. ^*Significant difference with cortisol; ^$Significant difference between mifepristone and relacorilant. The MI is expressed as the log₁₀-transformed normalized relative interaction, and statistical significance was calculated with t-test using R v.4.0.