TABLE 1.
Bacterial strains and plasmids used in this study
| Strain or plasmid | Relevant characteristic(s)a | Reference |
|---|---|---|
| Strains | ||
| E. coli DH5α | Fr− m+ φ80dlacZΔM15 | Gibco BRL |
| S. mitis | ||
| SF100 | Platelet-binding parental strain | 2 |
| PS344 | SF100ΔORF2-pblB | 2 |
| PS291 | SF100 pblA::pEVP3 with lacZ in the forward orientation | This study |
| PS294 | SF100 pblA::pEVP3 with lacZ in the reverse orientation | This study |
| Plasmids | ||
| pBluescript | Apr | Stratagene |
| pEVP3 | cat lacZ ori (E. coli) | 4 |
| pSF100AZ | pEVP3 with a BglII fragment of pblA in the same orientation as lacZ | This study |
| pSF100ArevZ | pEVP3 with a BglII fragment of pblA oriented opposite to lacZ | This study |
a
Apr, ampicillin resistance.