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. 2023 Jan 11;13:1088243. doi: 10.3389/fmicb.2022.1088243

FIGURE 1.

FIGURE 1

Characterization of mAb 2D12. (A) Identification of the reactivity of mAb 2D12 with CDV 851 strain in Vero and CDV NJ(11)2 in Vero-SLAM cells by immunofluorescence assay (IFA). The Vero cells were infected with CDV 851 strain, while Vero-SLAM cells were infected with CDV NJ(11)2 strain, which were inculated with mAbs 2D12 or G3N. (B) The purification of mAb 2D12. The mouse ascites of mAb 2D12 were purified by affinity chromatography using HiTrap TM Protein G and analyzed by SDS-PAGE. (C) Virus neutralization assay. The CDV 851 strain was incubated with the indicated dilution of purified mAb 2D12, the virus-antibody mixture was inoculated onto Vero cells, the cytopathic effect (CPE) was observed and the VN antibody titers expressed as the reciprocal of the highest plasma dilution giving 50% protection. Means ± S.D. of data from three independent experiments performed in triplicates are shown.