Table 2.
Estimation of protein amounts and number of molecules for GyrA and TopoI.
| Strain | ZnSO4 (μM) | Protein amount (ng) | Number of molecules | ||
|---|---|---|---|---|---|
| GyrA | TopoI | GyrA | TopoI | ||
| R6 | 0 | 21.6 ± 3.3 | 12.4 ± 2.1 | 2,046 ± 315 | 1,364 ± 225 |
| 75 | 22.5 ± 3.0 | 13.8 ± 1.7 | 2,133 ± 285 | 1,523 ± 187 | |
| 150 | 23.9 ± 2.5 | 12.4 ± 0.7 | 2,266 ± 241 | 1,359 ± 71 | |
| 300 | 24.1 ± 4.3 | 13.5 ± 2.0 | 2,290 ± 406 | 1,481 ± 219 | |
| PZntopA | 0 | 24.3 ± 6.0 | 12.2 ± 1.8 | 2,971 ± 734 | 1,726 ± 255 |
| 75 | 26.7 ± 4.5 | 20.2 ± 2.8 | 2,567 ± 435 | 2,250 ± 315 | |
| 150 | 28.4 ± 2.4 | 25.7 ± 3.7 | 2,739 ± 227 | 2,879 ± 411 | |
| 300 | 33.1 ± 5.7 | 35.9 ± 4.6 | 2,664 ± 459 | 3,352 ± 431 | |
| ΔtopAPZntopA | 0 | 16.7 ± 4.4 | 1.5 ± 0.2 | Nd | Nd |
| 75 | 20.1 ± 5.4 | 6.4 ± 1.2 | 1,555 ± 422 | 665 ± 109 | |
| 150 | 20.3 ± 6.1 | 8.3 ± 2.1 | 1,783 ± 532 | 848 ± 210 | |
| 300 | 24.0 ± 8.4 | 13.1 ± 4.9 | 1,961 ± 686 | 1,247 ± 460 | |