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. 2023 Jan 11;12:1044699. doi: 10.3389/fonc.2022.1044699

Figure 2.

Figure 2

GRP78 knockdown induces apoptosis in HNSCC cell lines. (A) Schematic drawing of the locations of the siRNAs targeting the coding sequence (CDS) or the 3’ untranslated region (UTR) of the GRP78 mRNA. (B) Western blot analysis of lysates from SCC15 cells transfected with si78 (CDS), si78 (3’UTR) or control siRNA (siCtrl) with a scrambled RNA sequence. The protein levels of GRP78, CHOP and cleaved-PARP (c-PARP) were determined, with GAPDH serving as loading control. (C) Quantitation of the relative levels of GRP78, CHOP and c-PARP after normalization with GAPDH serving as loading control. Data are presented as means ± S.D. (n=3). *P ≤ 0.05, **P ≤ 0.01, ***P ≤ 0.001 (Student’s t test). (D) Western blot analysis of lysates from SCC25 cells and (E) from SCC351 cells transfected with si78 (CDS) or control siRNA (siCtrl) for the indicated proteins.