Figure 3.

Treatment of GRP78 inhibitor YUM70 induces apoptosis and lowered cell viability of both cisplatin-sensitive and resistant HNSCC cell lines. (A) SCC15, SCC25, and SCC351 cells were treated with increasing doses of cisplatin, or PBS as a control, for 48 hr. Cell viability was determined by WST-1 assay. (B) Representative images of SCC15, SCC25, and SCC351 cells treated with the indicated concentrations of YUM70, or dimethyl sulfoxide (DMSO) as a control, for 48 hr. Scale bar = 50 μm. (C) Western blot analysis of lysates of SCC15, SCC25, and SCC351 cells treated with the indicated concentrations of YUM70 for 48 hr. The protein levels of GRP78, CHOP and cleaved-PARP (c-PARP) were determined, with GAPDH serving as loading control. (D) SCC15, SCC25, and SCC351 cells were treated with increasing doses of YUM70 or DMSO for 48 hr. Cell viability was determined by WST-1 assay. Data are presented as means ± S.D. (n=3).