Figure 8.

YUM70 treatment induces apoptosis in cisplatin-treated cisR-3 spheroids. (A) A representative image of cisR-3 spheroids. Scale bar = 200 μm. (B) GRP78 protein levels in cisR-3 grown in 2D culture and 3D spheroid culture was determined by Western blot with GAPDH serving as loading control. (C) Quantitative results of (B). (D) Western blot analysis of lysates of cisR-3 spheroids treated with the indicated dosages of cisplatin for 48 hr, alone or in combination with 10 μM YUM70. The protein levels of GRP78, CHOP, cleaved Caspase 7 (c-Cas7), and cleaved-PARP (c-PARP) were determined with GAPDH serving as loading control. (E-G) Quantitative results of (D) after normalization against the GADPH level. Data are presented as means ± S.D. (n=3). *P ≤ 0.05, **P ≤ 0.01, ns, not significant (Student’s t test).