FIGURE 5.

Novel proteins upregulated in fully differentiated primary human bronchial epithelial cells (phBECs) independent of disease state post-influenza virus infection. (A) Box plots of significantly upregulated novel proteins in fully differentiated phBECs on days 1 and 3 post-influenza virus infection (dpi). PLSCR1, phospholipid scramblase 1; HLA-F, human leukocyte antigen class I histocompatibility antigen, α chain F; CMTR1, cap-specific mRNA (nucleoside-2′-O-)-methyltransferase 1; DTX3L, E3 ubiquitin–protein ligase DTX3L; SHFL, shiftless antiviral inhibitor of ribosomal frameshifting protein. Box plots depict the median (middle horizontal line), the 25 and 75% percentile (lower and upper edge of the box), and the range between the largest and smallest value up to a maximum of 1.5 × interquartile range (vertical lines). Data points beyond that range are regarded as outliers and are shown as individual points. (B) mRNA expression of novel genes 1 and 3 dpi (PR8/mock). For some COPD phBECs samples, the obtained amount of RNA was insufficient to cover all qPCR reactions, restricting the COPD-derived samples to n = 3 and n = 2 on 1 dpi. Symbol legend (in analogy to panels A and C): Cyan squares, ctrl samples; red squares, COPD samples. (C) Western blots of PLSCR1, HLA-F, and DTX3L. VCL (vinculin) was used as a loading control. Irrelevant parts of the Western blot images were cropped to only present the areas of interest. Uncropped Western blot images are given in Supplementary Figure 9.