Figure 6.

Knock-down of bcPRKX enhanced the cells’ resistance against SVCV. (A) HEK 293T cells in a 6-well plate were co-transfected with Flag-bcPRKX and pLKO.1-sh-bcPRKX-1/2/3 ( Table 1 ) (or pLKO.1-sh-scramble) respectively. The cells were lysed for immunoblotting at 24 h post-transfection. The numbers represented the densities of bcPRKX bands (normalized to β-actin bands). (B, C) MPK cells in a 6-well plate were transfected with pLKO.1-sh-scramble or pLKO.1-sh-bcPRKX-1 and the cells were infected with SVCV at 24 h post-transfection. The media of the cells were harvested for the viral titration at 48 h post-transfection and the cells were used for RNA isolation and qPCR analysis. Asterisk (*) stands for statistical difference (p<0.05).