Table 1.

Plasmids used in this study.

Plasmid	Application
Overexpression
pcDNA5/FRT/TO-Flag-bcPRKX pcDNA5/FRT/TO-HA-bcPRKX pcDNA5/FRT/TO-Myc-bcPRKX pcDNA5/FRT/TO-Flag-bcTAK1 pcDNA5/FRT/TO-HA-bcTAK1 pcDNA5/FRT/TO-HA-bcIRF7 pcDNA5/FRT/TO-HA-Ub	Immunoblotting, Immunofluorescence Microscopy, Luciferase report assay, Plaque assay and Quantitative Real-time PCR
Luciferase Reporter Assay
Luci-bcIFNa Luci-DrIFNϕ1 Luci-DrIFNϕ3 Luci-NF-κB pRL-TK	Black Carp IFNa, Zebrafish IFNϕ1/3 and Human NF-κB Promoter Activity Analysis
Knock-Down
pLKO.1-sh-scramble pLKO.1-sh-bcPRKX-1 pLKO.1-sh-bcPRKX-2 pLKO.1-sh-bcPRKX-3 pLKO.1-sh-bcPRKX-4	Knock-Down bcPRKX in MPK cells