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. 2022 Aug 4;73(1):136–143. doi: 10.1016/j.identj.2022.06.025

Fig. 2.

Fig 2

Circ_0099630 repressed iPDLC proliferation and osteogenic differentiation. A, RT-qPCR demonstrated the transfection efficiency of sh-circ_0099630 and circ_0099630 overexpression plasmid. B–G, Effects of circ_0099630 overexpression and knockdown on iPDLC viability, proliferation, and cell cycle progression were determined using MTT, EdU, and flow cytometry assays. H–J, ALP staining, ARS staining, ALP activity, and ARS quantification were carried out to verify the effects of circ_0099630 overexpression and knockdown on iPDLC osteogenic differentiation. K and L, WB detection of the effects of circ_0099630 overexpression and knockdown on OSX, ALP, and RUNX2 protein levels in iPDLCs.

**P < .01 and ***P < .001.

ALP, alkaline phosphatase; ARS, alizarin red S; EdU, 5-ethynyl-2’-deoxyuridine; iPDLC, inflamed periodontal ligament cell; MTT, 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; OSX, osterix; RT-qPCR, reverse transcription  quantitative polymerase chain reaction; WB, Western blotting.