FIG. 1.

Schematic diagram of the ireA gene and the flanking sequence described in this report. (Left to right) (i) The DNA sequence beginning 317 bases from the 3′ end of ireA possesses 34 to 44% identity and 50 to 61% similarity to defined or probable transposases from a wide variety of bacteria, (ii) the ireA gene, a putative Fur DNA binding site, was present 5′ to ireA (bases −27 to minus −45), (iii) 2.7 kb of DNA that does not possess any DNA or protein homology to known sequences, (iv) 2.7 kb 5′ to the start of ireA is a DNA sequence whose putative protein product possesses 44% identity and 48% similarity to the first 337 aa of a transposase that was recently described for the E. coli Nissle 1917 fimB gene. The arrows below the solid line indicate the direction of transcription for ireA and the transposase homologues.