TABLE 1.
The top 15 miRNAs overexpressed (fold change > 2; adjusted P < 0.05) in K/VP.5 compared with K562 cells. Fold change determined from miRNA-seq data (GEO accession number: GSE141687; Kania et al., 2020) are listed below. The DIANA-microT-CDS algorithm (Paraskevopoulou et al., 2013) revealed that the TOP2β/180 3′-UTR harbored MREs for seven (bolded) of the 15 overexpressed miRNAs.
| miRNA | Fold Expression (K/VP.5)/(K562) | Adjusted P Value |
|---|---|---|
| hsa-miR-9-3p | ∼75 | 6.8 × 10−5 |
| hsa-miR-372-3p | ∼45 | 3.5 × 10−4 |
| hsa-miR-7706 | ∼32 | 2.3 × 10−3 |
| hsa-miR-504-3p | ∼28 | 8.5 × 10−8 |
| hsa-miR-383-5p | ∼27 | 6.0 × 10−4 |
| hsa-miR-9-5p | ∼26 | 9.2 × 10−19 |
| hsa-miR-451a | ∼16 | 4.6 × 10−5 |
| hsa-miR-493-5p | ∼16 | 3.6 × 10−10 |
| hsa-miR-675-5p | ∼16 | 1.9 × 10−10 |
| hsa-miR-127-3p | ∼15 | 4.4 × 10−8 |
| hsa-miR-196a-5p | ∼15 | 1.0 × 10−15 |
| hsa-miR-543-3p | ∼14 | 6.6 × 10−4 |
| hsa-miR-3681-5p | ∼13 | 5.0 × 10−10 |
| hsa-miR-493-3p | ∼13 | 3.4 × 10−7 |
| hsa-miR-1299-3p | ∼13 | 2.1 × 10−3 |