KEY RESOURCES TABLE.

REAGENT or RESOURCE	SOURCE	IDENTIFIER

Biological samples

Fresh bovine eyes	Mahan Packing Company	N/A

Chemicals, peptides, and recombinant proteins

Acetonitrile	Millipore Sigma	Cat# 1000291000
Ammonium bicarbonate	Sigma-Aldrich	Cat# 5330050050
Graphene oxide	Sigma-Aldrich	Cat# 763705-100ML
Iodoacetamide	GE Healthcare	Cat# RPN6302
Trypsin/Lys-C Mix	Promega	Cat# V5073

Deposited data

Raw cryo-EM data	This paper	EMPIAR-11251 https://doi.org/10.6019/EMPIAR-11251
Cryo-EM structure and map of GS	This paper	PDB ID: 7U5N EMDB ID: EMD-26356 https://doi.org/10.2210/pdb7U5N/pdb
Cryo-EM structure and map of GAPDH	This paper	PDB ID: 7U5M EMDB ID: EMD-26355 https://doi.org/10.2210/pdb7U5M/pdb
Cryo-EM structure and map of FT	This paper	PDB ID: 7U5L EMDB ID: EMD-26354 https://doi.org/10.2210/pdb7U5L/pdb
Cryo-EM structure and map of DPYSL2	This paper	PDB ID: 7U5K EMDB ID: EMD-26353 https://doi.org/10.2210/pdb7U5K/pdb
Cryo-EM structure and map of FPA	This paper	PDB ID: 7U5J EMDB ID: EMD-26352 https://doi.org/10.2210/pdb7U5J/pdb
Cryo-EM structure and map of MtCK	This paper	PDB ID: 7U5I EMDB ID: EMD-26351 https://doi.org/10.2210/pdb7U5I/pdb
Cryo-EM structure and map of DNPEP	This paper	PDB ID: 7U5H EMDB ID: EMD-26350 https://doi.org/10.2210/pdb7U5H/pdb
Raw mass spectrometry proteomics data	This paper	PXD037186 (Table S3)

Software and algorithms

Coot	Emsley et al.¹¹⁸	https://www2.mrc-lmb.cam.ac.uk/personal/pemsley/coot/
cryoSPARC v3	Punjani et al.¹¹⁹	https://cryosparc.com/
MaxQuant	Cox and Mann	https://www.maxquant.org/
Phenix	Adams et al.¹²⁰	https://phenix-online.org/
STRING database	STRING	https://string-db.org/
UCSF-Chimera	Pettersen et al.¹²¹	https://www.cgl.ucsf.edu/chimera/
UCSF-Chimera X	Pettersen et al.¹²²	https://www.cgl.ucsf.edu/chimerax/

Other

C18 Microspin column	Nest Group	Cat# SEM SS18V
Quantifoil R 2/2 Cu 200 mesh, copper	Quantifoil	Cat# Q210CR2