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. 2023 Jan 25;23:55. doi: 10.1186/s12870-023-04062-9

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© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — IPA1 directly binds the promoter of SNAC1. A Segmented simplified map of the target genes; sequence is GTAC. B Interactions between IPA1 and the promoter fragments of SNAC1, shown with a yeast one-hybrid assay. C The vector used in dual luciferase assays. D Detection of the luciferase activity of reporter genes. E Segmented simplified map of the target genes; sequence represents the GTAC motif. Sequences of segments: A1 and A2; probe sequence: probe-probe4; probe competitor: competitor; mutation probe: mutation probe; red bases: mutant bases. F Results of EMSA detection of IPA1 and SNAC1 promoters