Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Jan 25;21:45. doi: 10.1186/s12967-023-03890-5

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2023

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

PMC Copyright notice

Fig. 2 — Trajectory of epithelial cell deterioration. A UMAP clustering of epithelial cells (n = 11,635) from scRNA-seq of patients with CRC. B Expression of marker genes in seven clusters of epithelial cells. C, D Potential trajectory of epithelial cell deterioration in adenoma and carcinoma tissues (n = 3688) inferred using Monocle2 based on gene expression. Pseudotime is shown numbered 1- 6 and the red dash indicates the direction of the pseudotime. E, F The expression levels of APCDD1 and MMP7 in different pseudotime of epithelial cell subpopulation. The average gene expression is indicated by the black dash. G The heatmap shows dynamic changes in gene expression, including benign epithelial markers, mutant genes associated with epithelial cell deterioration, epithelial apoptotic processes, and cell proliferation. H The heatmap shows expression of cell type-specific gene markers in different epithelial cell clusters. Marker genes for each cluster identified by Seurat analysis, with four genes selected for each cluster highlighted at the top