FIG. 2.

Plasmid maps of cat promoter probe vector pAM2355 and operon fusion plasmids pAM2364 (lkt::cat) and pAM2365 (lapT::cat). Plasmid pAM2355 contains transcriptional terminators on either side of the cat reporter gene to prevent readthrough from other sites on the plasmid. A PCR-amplified fragment containing the lapT/lktC promoter region was ligated upstream of the promoterless cat gene in both orientations to create pAM2364 and pAM2365. All of the plasmids contain the M. haemolytica origin of replication from pYFCI. Only relevant restriction sites are shown.