TABLE 1.
Strains, plasmids, and oligonucleotides used in this study
| Strain, plasmid, or oligonucleotide | Description | Reference or source |
|---|---|---|
| Strains | ||
| SH1217 | M. haemolytica A1, Sms | 10 |
| XLI-Blue | E. coli recA1 endA1 gyrA96 thi-1 hsdR17 supE44 relA1 lac [F′ proAB lacIqZΔM15 Tn10] | Stratagene |
| SH2020 | SH1217 Φ(lktC::cat)2 | This study |
| SH2410 | SH1217 Φ(lktC::cat)3 SspI | This study |
| SH2405 | SH1217 Φ(lktC::cat)4 Sau3A | This study |
| Plasmids | ||
| pYFC1 | Native M. haemolytica plasmid, Smr | |
| pSH224 | pBlueScript KSII+::7-kb PstI (lapCABT lktCA′) | 19 |
| pNF2237 | pYFCI::Φ(lktC::cat)1 | 9 |
| pNF2283 | pYFC1::cat (promoterless) | 10 |
| Oligonucleotides | ||
| SH48 | 5′-cctaaaccatcacc-3′ | This study |
| SH141 | 5′-gcgcggatccgtcgacataaaaagtccctgtgttttcattataagg-3′ | This study |
| SH142 | 5′-cctaatttagaataattatcgagtgcaaattatgaatcccgggatccgccg-3′ | This study |
| SH186 | 5′-cgatgccattgggatatatcaacggtgg-3′ | This study |