FIGURE 2.

Adipocytes were dedifferentiated by the interaction with GC cells. (A) Western blot analysis of the protein expression level of adiponectin in single‐cultured adipocytes (NC) and co‐cultured adipocytes with GC cells. The protein expression level of adiponectin was decreased in 96 h co‐cultured adipocytes. (B–D) Single‐cultured adipocyte stained with BODIPY and Hoechst 33342. (B′–D′) Adipocytes co‐cultured with MKN45 stained with BODIPY and Hoechst 33342. (B″‐D″) Adipocytes co‐cultured with OCUM stained with BODIPY and Hoechst 33342. The number and size of BODIPY‐positive lipid droplets were decreased in the adipocytes co‐cultured with GC cells for 96 h. (E) Real‐time PCR of adipocyte markers (adiponectin, C/EBPα, PPARγ, and Glut4) and pre‐adipocyte marker (Glut1) in single‐cultured adipocytes (NC) and co‐cultured adipocytes with GC cells for 96 h. The expression levels of adipocyte markers were decreased, and the expression level of pre‐adipocyte marker Glut1 was increased in the adipocytes co‐cultured with GC cells for 96 h (n = 3, mean ± SEM; two‐tailed Student's t‐test, *p < 0.05, **p < 0.01 and ***p < 0.001). Scale bar: 200 μm (B–D″)