KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Goat polyclonal SOX2 (Y-17) | Santa Cruz Biotechnology | Cat# sc-17320; RRID: AB_2286684 |
| Rabbit polyclonal anti-BLBP | Millipore | Cat#ABN14; RRID: AB_2494022 |
| Mouse monoclonal anti-GFAP | Millipore | Cat# MAB36014; RRID: AB_11212597 |
| Rabbit polyclonal anti-GFAP | Agilent | Cat# Z0334; RRID: AB_10013382 |
| Rabbit polyclonal SOX10 | Abcam | Cat# ab27655; RRID: AB_778021 |
| Goat polyclonal anti-SOX9 | R&D system | Cat# AF3075; RRID: AB_2194160 |
| Mouse polyclonal anti-SPARCL1 | R&D system | Cat# AF2836; RRID: AB_2195097 |
| Chicken polyclonal anti-GFP | Abcam | Cat# ab13970; RRID: AB_300798 |
| Rabbit polyclonal anti-Kir4.1 | Alomone labs | Cat# APC-035; RRID: AB_2040120 |
| Rabbit polyclonal anti-GLT1 | Provided by J.D. Rothstein | Higashimori et al., 2016 |
| Guinea pig polyclonal anti-VGLUT1 | Millipore | Cat# AB5905; RRID: AB_2301751 |
| Rabbit monoclonal anti-S100beta | Abcam | Cat#ab52642 RRID: AB_882426 |
| Mouse monoclonal anti-PSD95 | Synaptic Systems | Cat# 124011; RRID: AB_10804286 |
| Mouse monoclonal anti-NeuN | Millipore | Cat# MAB377; RRID: AB_2298772 |
| Rabbit polyclonal c-Fos | Santa Cruz Biotechnology | Cat# sc-52; RRID: AB_2106783 |
| Rabbit polyclonal Anti-GLAST | Proteintech | Cat# 20785-1-AP; RRID: AB_2878738 |
| Mouse monoclonal β-actin | Cell Signaling Technology | Cat# 3700; RRID: AB_2242334 |
| Rabbit monoclonal anti-GAPDH | Cell Signaling Technology | Cat# 2118; RRID: AB_561053 |
| HRP goat anti-rabbit IgG (H + L) | Thermo Fisher Scientific | Cat# 31460; RRID: AB_228341 |
| HRP goat anti-mouse IgG (H + L) | Thermo Fisher Scientific | Cat# 31430; RRID: AB_228307 |
| Normal goat IgG control | R&D Systems | Cat# AB-108-C; RRID: AB_354267 |
| Alexa Fluor® 488 AffiniPure F(ab’)2 Fragment Donkey Anti-Goat IgG (H + L) | Jackson ImmunoResearch Laboratories | Cat#705-546-147; RRID: AB_2340430 |
| Alexa Fluor® 594 AffiniPure F(ab’)2 Fragment Donkey Anti-Goat IgG (H + L) | Jackson ImmunoResearch Laboratories | Cat# 705-586-147; RRID: AB_2340434 |
| Alexa Fluor® 488 AffiniPure F(ab’)2 Fragment Donkey Anti-Rabbit IgG (H + L) | Jackson ImmunoResearch Laboratories | Cat# 711-546-152; RRID: AB_2340619 |
| Alexa Fluor® 488 AffiniPure F(ab’)2 Fragment Donkey Anti-Mouse IgG (H + L) | Jackson ImmunoResearch Laboratories | Cat# 715-546-150; RRID: AB_2340849 |
| Alexa Fluor® 594 AffiniPure F(ab’)2 Fragment Donkey Anti-Mouse IgG (H + L) | Jackson ImmunoResearch Laboratories | Cat# 715-586-150; RRID: AB_2340857 |
| Biotin-SP (long spacer) AffiniPure F(ab’)2 Fragment Donkey Anti-Goat IgG (H + L) | Jackson ImmunoResearch Laboratories | Cat# 705-066-147; RRID: AB_2340398 |
| Alexa Fluor® 594 AffiniPure F(ab’)2 Fragment Donkey Anti-Guinea pig IgG (H + L) | Jackson ImmunoResearch Laboratories | Cat# 706-586-148; RRID: AB_2340475 |
| Alexa Fluor® 488 AffiniPure F(ab’)2 Fragment Donkey Anti-Chicken IgG (H + L) | Jackson ImmunoResearch Laboratories | Cat# 703-546-155; RRID: AB_2340376 |
| Sheep Anti-Digoxigenin Fab fragments Antibody, AP Conjugated | Roche | Cat# 11093274910; RRID: AB_514497 |
| N-PER™ Neuronal Protein Extraction Reagent | Thermo Fisher | Cat# 87792 |
| Chemicals, peptides, and recombinant proteins | ||
| Tamoxifen | Sigma-Aldrich | Cat# T5648 |
| 4-hydroxytamoxifen | Sigma-Aldrich | Cat# H7904 |
| Sunflower seed oil | Sigma-Aldrich | Cat# 47123 |
| 5-Ethynyl-2′-deoxyuridine (EDU) | Thermo Fisher Scientific | Cat# A10044 |
| 10x PBS | K-D Medical | Cat# RGF-3210 |
| Paraformaldehyde | Electron Microscopy Science | Cat# 1570-S |
| O.C.T. compound | VWR International | Cat# 361603E |
| Normal donkey serum | Jackson ImmunoResearch Laboratories | Cat# 017-000-121 |
| DAPI | Sigma-Aldrich | Cat# D9542 |
| Xylene | Sigma-Aldrich | Cat# 534056 |
| Mounting medium | Thermo Fisher Scientific | Cat# SP15-100 |
| Poly-L-lysine | Sigma-Aldrich | Cat# P4707 |
| laminin | Sigma-Aldrich | Cat# L2020 |
| High-glucose DMEM medium | Gibco | Cat# 11965-092 |
| BSA | Cell Signaling Technology | Cat# 9998 |
| Penicillin/Streptomycin | Thermo Fisher Scientific | Cat# 15140122 |
| Trypsin-EDTA | Promega | Cat# V528A |
| Fetal bovine serum | Sigma-Aldrich | Cat# 12306-C |
| HBSS | Sigma-Aldrich | Cat# 55021C |
| Ketamine | VetOne | Cat# 13985-584-10 |
| Xylazine | AnaSed Injection | Cat# 59399-110-20 |
| Sulforhodamine 101 | Sigma-Aldrich | Cat# S7635 |
| Tarichatoxin | Sigma-Aldrich | Cat# T8024 |
| Picrotoxin | Sigma-Aldrich | Cat# P1675 |
| Protease and phosphatase inhibitor cocktail | Thermo Fisher | Cat# PPC1010 |
| 10X Tris/Glycine/SDS buffer | BIO-RAD | Cat# 1610772 |
| Tween 20 | Sigma-Aldrich | Cat# T8787 |
| Triton X-100 | Sigma-Aldrich | Cat# P9416 |
| AstroMACS Medium | Miltenyi Biotec | Cat# 130-117-031 |
| MACS® Neuro Medium | Miltenyi Biotec | Cat# 130-093-570 |
| L-Glutamine Solution 200 mM | Sigma-Aldrich | Cat# 56-85-9 |
| NBT/BCIP stocking solution | Roche | Cat# 11681451001 |
| PMSF | Cell Signaling Technology | Cat# 8553 |
| QIAzol Lysis Reagent | QIAGEN | Cat# 79306 |
| Glutamate | Sigma | Cat# PHR1007 |
| Streptavidin, Pacific Blue™ conjugate | Thermo Fisher Scientific | Cat# S11222 |
| Critical commercial assays | ||
| BCA protein assay Kit | Thermo Fisher Scientific | Cat# 23225 |
| Western Lightening Plus ECL | Perkin Elmer | Cat# NEL103001EA |
| Commercial glutamate assay Kit | Sigma-Aldrich | Cat# MAK004 |
| NEBNext Ultra Directional RNA Library Prep Kit | New England BioLabs Inc | Cat# E7420 |
| DIG RNA labeling Kit | Roche | Cat# 11175025910 |
| RNeasy Lipid Tissue Mini Kit | Qiagen | Cat# 74804 |
| RNase-Free DNase Set | Qiagen | Cat# 79254 |
| Qiagen Omniscript RT Kit | Qiagen | Cat# 205111 |
| QuantiTect SYBR® Green PCR Kit | Qiagen | Cat# 204145 |
| SimpleChIP Enzymatic Chromatin IP Kit | Cell Signaling Technologies | Cat# 9003 |
| HNPP/Fast Red Fluorescent Detection set | Roche | Cat# 11758888001 |
| Neural Tissue Dissociation Kit (P) | Miltenyi Biotec | Cat# 130-092-628 |
| Anti-ACSA-2 MicroBead Kit | Miltenyi Biotec | Cat# 130-097-678 |
| Trans-blot turbo RTA midi 0.2 μm nitrocellulose transfer Kit | BIO-RAD | Cat# 1704271 |
| Deposited data | ||
| RNA-seq | Guo Lab | Datebase: GSE182032 |
| ChIP-seq | Zhang Lab | Datebase: GSE85213 |
| ChIP-seq | Berninger Lab | Datebase: GSE96539 |
| Experimental models: Organisms/strains | ||
| B6; FVB-Tg(Aldh1l1-cre/ERT2)1Khakh/J | Jackson Laboratory | RRID: IMSR_JAX:029,655 |
| B6.Cg-Tg(Gfap-cre)77.6Mvs/2J | Jackson Laboratory | RRID: IMSR_JAX:024098, |
| Sox2tm1.1Lan/J | Jackson Laboratory | RRID: IMSR_JAX:013,093 |
| B6.129X1-Gt(ROSA)26Sortm1(EYFP)Cos/J | Jackson Laboratory | RRID: IMSR_JAX:006,148 |
| B6.129(Cg)-Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J | Jackson Laboratory | RRID: IMSR_JAX:007,676 |
| Oligonucleotides | ||
| See Table S7 | ||
| Software and algorithms | ||
| ImageJ | ImageJ public | https://imagej.net/Fiji/Downloads |
| IMARIS version 9.30 | Oxford instruments | https://imaris.oxinst.com/newrelease |
| MEME-CHIP Version 5.4.1 | Noble research lab | http://meme-suite.org/tools/meme-chip |
| GREAT Version 3.0 | Bejerano Lab | http://great.stanford.edu/great/public-3.0.0/html/ |
| DESeq2 R package vesion 2.1.6.3 | Bioconductor | https://bioconductor.org/packages/release/bioc/html/DESeq2.html |
| ClusterProfiler R package version 2.34.3 | Bioconductor | https://bioconductor.org/packages/release/bioc/html/clusterProfiler.html |
| CatWalk XT | Noldus Information Technology | https://www.noldus.com/catwalk-xt |
| Photobeam Activity System | San Diego instrument | https://sandiegoinstruments.com/product/pas-open-field/ |
| Ethovision tracking software system vesion XT.14 | Noldus | https://www.noldus.com/ethovision-xt |
| SmartCageTM system | AfaSci, Inc. | https://www.afasci.com/index.php/instruments/smartcage |
| Minianalysis 6.0.7 software | Synaptosoft | http://www.synaptosoft.com/MiniAnalysis/ |
| GraphPad Prism version 8.0 | GraphPad Software | https://www.graphpad.com/company |
| Bioanalyzer 2100 system | Agilent Technologies | https://www.agilent.com |