Table 1.

Controversies and Directions for Future Study

•Is transnitrosylation the major transport system for NO between proteins in the cell or among cells?

•Protein affinity labels coupled to MS detection of the S-nitrosoproteome to discover new transnitrosylation networks – are these hidden networks abundant?

•Bioinformatic approaches to uncover hidden transnitrosylation networks

•Why are some S-nitrosylated proteins upregulated while others are downregulated in disease states?

•Why are S-nitrosylated proteins differentially expressed in male and female human brain, particularly in disease states?

•Futuristic denitrosylating therapeutic drugs. Can such drugs be ‘directed’ or ‘targeted’ to a specific S-nitrosylated protein?