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Comparison of pulmonary TNF-α, IFN-γ, and MIP-1α gene expression in WT mice infected intratracheally (C. neoformans 52D or C. neoformans 145A; 104 CFU). Total lung RNA was isolated from the uninfected mice (U) on day 7 postinoculation. Expression of cytokine mRNA was analyzed via RT-PCR. The cytokine PCR products were confirmed by Southern blotting with specific DNA probes.
