FIG. 3.
Quantitative ELISA analysis of IFN-γ secretion by murine lymphocytes isolated after DNA immunization (pCI, pCIBFR, or pCIP39) and subsequent stimulation in culture using different antigens. Splenocytes from the immunized mice (four mice/group) were harvested at week 3 (A) and 12 (B) after the last immunization. Assays were performed on supernatants of 2 × 106 lymphocytes per ml. Lymphocytes were restimulated with the indicated antigens. At 96 h after restimulation, supernatants were analyzed for IFN-γ levels. Samples were tested in duplicate.