Fig 2. Effect of buffer additives on DNase I activity and eGFP concentration.

DNase I reactions were prepared that comprised RPP30 gBlock and AAV2 with different additives. Samples were incubated at 37°C for 30 min and then serially diluted into the ddPCR concentration range with polyA buffer. Capsids were lysed at 95°C for 10 min prior to assembling ddPCR reactions. (A) RPP30 concentration without and with DNase I. The estimated RPP30 concentration for samples containing DNase I is indicated with numerical values because the bars are not visible. (B) eGFP concentration with DNase I digestion. Error bars represent the 95% confidence interval.