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. 2023 Jan 25;18(1):e0280242. doi: 10.1371/journal.pone.0280242

Fig 3. Buffer effects on the viral genome ITR2 concentration.

Fig 3

Prior to droplet formation, ddPCR reactions were prepared using a single-stranded viral vector, AAV2, that was diluted into the ddPCR range with either polyA buffer or PCR buffer and lysed for 10 min at 95°C before being used as the template for ddPCR. Two separate duplex reactions with 5 U MspI were prepared: ITR2/eGFP and SV40/eGFP. (A) The concentration from the ITR (black bar) and eGFP (black striped bar) duplex reaction is shown. The concentration from the SV40 and eGFP duplex reaction is indicated by the gray and gray striped bars respectively. (B) The concentration ratio of ITR2 to eGFP (black bars) and SV40 to eGFP (gray bars) is indicated. The error bars represent the 95% confidence interval.