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. 2023 Jan 25;18(1):e0280242. doi: 10.1371/journal.pone.0280242

Fig 4. Effect of buffer composition on the viral genome concentration.

Fig 4

Seven different dilution buffer formulations were used to dilute AAV2 after DNase I digestion. Buffer identities are listed in S1 Text. Buffers 1‒3 are based on DNA suspension buffer and buffers 4‒7 are based on PCR buffer. For reference, buffer 1 is polyA buffer and buffer 7 is PCR buffer. Duplex reactions with ITR2 FAM and eGFP HEX were analyzed. (A) The concentration of the ITR (black bars) and eGFP (gray bars). (B) The concentration ratio of ITR2 to eGFP. The error bars represent the 95% confidence interval.