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PolyA buffer and PBS with 100 ng/μL polyadenylic acid (PBS + pA) were prepared with Pluronic F-68 concentrations that varied from 0–0.1% and used to dilute AAV2 after DNase I digestion. Duplex reactions with ITR2 FAM and eGFP HEX were analyzed. (A) The concentration of the ITR (black bars) and eGFP (gray bars). (B) The concentration ratio of ITR2 to eGFP. The error bars represent the 95% confidence interval.
