Fig 7. Effect of proteinase K on AAV5.

After DNase I digestion, AAV5 was incubated either without (no PK) or with (PK) proteinase K for 2 hours at 50°C and then further processed as described in the Materials and Methods. The two samples were serially diluted in parallel with polyA+ buffer and either added directly to the ddPCR supermix (no capsid lysis step) or had the capsid thermally lysed (with capsid lysis step) prior to adding to the ddPCR supermix. The ITR2 concentration (black bars) and eGFP concentration (gray bars) is shown. The values indicate the ITR2/eGFP concentration ratio. The error bars and numerical errors represent the 95% confidence interval.