Skip to main content
. 2023 Jan 25;18(1):e0280242. doi: 10.1371/journal.pone.0280242

Fig 10. Milepost experiment using pAV-CMV-GFP.

Fig 10

Plasmid pAV-CMV-GFP was used as a template for duplex ddPCR reactions using CMV-Enh FAM and the indicated HEX assay. (A) Schematic showing approximate distances from the end of the CMV-Enh amplicon (blue) and the beginning of the HEX amplicons (green). Reactions were prepared with 5 U of (B) HindIII or (C) MspI. The concentration of CMV-Enh (black bar), the HEX assay (gray bar), and linkage concentration (white bar) is shown with the corresponding standard deviations. The calculated linkage percentage between CMV-Enh FAM and the corresponding duplexed HEX assay is indicated.