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An AAV2 sample was DNase I treated and lysed for 10 min at 95°C prior to droplet formation (pre-droplet lysis) and then used as a template for duplex ddPCR reactions using CMV-Enh FAM and the indicated HEX assay with either no enzyme, MspI, MseI, or a double digest with MspI and MseI. The (A) calculated linkage percentage and (B) concentration ratio of CMV-Enh FAM to the HEX assay with the corresponding standard deviations are shown.
