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(A) AAV2 and (B) AAV5 samples were either lysed in droplets (in drop) or prior to droplet formation for 1, 2.5, 5, 10, or 15 minutes at 95°C and used as a template for a milepost analysis using CMV-Enh FAM and the indicated HEX assay. The error bars represent the standard deviations.
