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. 2023 Jan 25;18(1):e0280242. doi: 10.1371/journal.pone.0280242

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© 2023 Prantner, Maar

This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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Fig 13 — The viral vector AAV2 was lysed prior to droplet formation for 1 minute at 95°C and then used as a template for a milepost experiment using CMV-Enh_s-FAM as the anchor assay and either CMV-Pro, eGFP, GFP-L or SV40 as the HEX assay. The linkage percentage was calculated using equations based on an average concentration for the FAM and HEX assays (average) or equations that compensated for differences in assay concentration between the FAM and HEX assay (compensated). The error bars represent the standard deviation and are smaller than the marker when not visible.