Fig. 5. SFII showed an inhibitory effect on AAI induced acute injury and fibrosis in the kidney of adult mice.

a Schematic representation of the three-days treatment in different groups of adult mice. Mice were intraperitoneally (i.p.) injection with NaHCO₃ (gray arrow) or AAI (red arrow, 5 mg/kg) for three consecutive days as the NaHCO₃ group or AAI group. The SFII treatment group was administered (oral) with SFII (orange arrow) 12 h before injecting AAI and at the time of injection. b The levels of blood CRE in different groups after three-day treatment. c Representative images of H&E staining (scale bars, 500 or 100 μm), TUNEL staining (scale bars, 500 or 100 μm), and Sirius red staining (scale bars, 500 or 100 μm) of kidney samples in different treatment groups. The black arrows point to apoptosis cells in the kidney. d The staining area of kidney fibrosis was calculated. e The percentage of renal cell apoptosis was calculated. f Western blot analysis to evaluate the AAI-induced kidney injury in adult mice treated with or without SFII. g Design of SFII administration in renal fibrosis model of adult mice. Mice were injected (i.p.) with a single dose of NaHCO₃ (gray arrow) or AAI (red arrow, 10 mg/kg) as the NaHCO₃ group or AAI group. The SFII treatment group received SFII (orange arrow) 12 h before AAI administration and three consecutive days after a single dose of AAI. Mice were sacrificed 14 days after a single dose of NaHCO₃ or AAI. h The level of blood CRE and UREA in different treatment groups. i Representative images of H&E staining (scale bars, 500 or 100 μm) and Sirius red staining (scale bars, 500 or 100 μm) of kidney samples in different treatment groups. *P < 0.05, **P < 0.01, ***P < 0.001.