Fig. 2. tSNE map of individual cell types from mouse TG.

Mice were infected with HSV-1 McKrae. TG were harvested 35 days PI, pooled together (30 TG), and dissociated to single cells. Dead cells were removed using a dead cell removal kit, and CD45⁺ immune cells were isolated as described in Materials and Methods. Purified cells were mixed back with the original single-cell suspension for single-cell sequencing. ImmGen reference was used to determine immune cell types, while multiple mouse single-cell sequence references were used to determine neurons.