Key resources table.
| Reagent type (species) or resource | Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Mus musculus, C57BL/6J) | WT | Jax and Vance Lab colony, Jax stock No. 000664 | ||
| Strain, strain background (Mus musculus, C57BL/6J) | Nlrc4–/– | Vance Lab colony Tenthorey et al., 2020 | Crossed to 129. Nlrc4–/– mice for mapping studies | |
| Strain, strain background (Mus musculus, C57BL/6J) | Casp11–/– | Vance Lab colony, this paper | ||
| Strain, strain background (Mus musculus, C57BL/6J) | Il1r1–/– | Jax and Vance Lab colony, Jax stock No. 003245 | ||
| Strain, strain background (Mus musculus, C57BL/6J) |
Casp1/11/8–/–
Ripk3–/– |
Vance Lab colony Rauch et al., 2017 | ||
| Strain, strain background (Mus musculus, C57BL/6J and C57BL/6N mixed) | Rosa26LSL-Nlrc4 (formerly called iNlrc4) | Vance Lab colony Rauch et al., 2017 | Encode a Cre-inducible Nlrc4 gene in the Rosa26 locus | |
| Strain, strain background (Mus musculus, C57BL/6J) | Lyz2Cre | Jax and Vance Lab Colony, Jax stock No. 004781 | ||
| Strain, strain background (Mus musculus, 129S1/SvImJ) | WT | Jax and Vance Lab colony, Jax stock No. 002448 | ||
| Strain, strain background (Mus musculus, 129S1/SvImJ) | Nlrc4–/– | Vance Lab colony Mitchell et al., 2020 | Crossed to B6. Nlrc4–/– mice for mapping studies | |
| Strain, strain background (Shigella flexneri serovar 2a) | WT 2457T | Lesser Lab | Streptomycin resistant | |
| Strain, strain background (Shigella flexneri serovar 2a) | ΔospC3 2457T | Lesser Lab Mou et al., 2018 | Streptomycin resistant | |
| Antibody | Rat anti-mIL-1β capture and goat anti-mIL-1β polyclonal detection antibodies | R&D | DY401 | For ELISA (each used at 100 µL per well) |
| Antibody | Rat anti-mCXCL1 capture and rat anti-mCXCL1 detection antibodies | R&D | DY453 | For ELISA (each used at 100 µL per well) |
| Antibody | Goat anti-mMPO capture and goat anti-mMPO detection antibodies | R&D | DY3667 | For ELISA (each used at 100 µL per well) |
| Antibody | Monoclonal anti-TNFα capture and detection antibodies | Thermo Fisher | BMS607HS | For ELISA. Capture antibody is precoated on purchased plates, detection antibody used at 50 µL per well |
| Antibody | Hamster anti-TNFα monoclonal neutralizing antibody | Bio X cell | TN3-19.12 | In vivo treatments, 200 μg daily |
| Antibody | Polyclonal Armenian hamster IgG isotype control | Bio X cell | BE0091 | In vivo treatments,, 200 μg daily |
| Antibody | Rat anti-mCasp11 monoclonal antibody | Novus | 17D9 | 1:500 |