Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2023 Jan 11;13:1113237. doi: 10.3389/fmicb.2022.1113237

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

Copyright © 2023 Goffredi, Appy, Hildreth and deRogatis.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

PMC Copyright notice

Fluorescent visualization and localization of Vibrio in (A) the parasitic isopod Elthusa vulgaris. (B) Ventral dissection showing the enlarged digestive ceca. Scale bar 5 mm. (C) Excised digestive ceca and intestine. Scale bar, 4 mm. A-C were taken with a Pentax WG-III handheld camera. (D) intestine sectioned and Wright stained. Scale bar, 1 mm. Square indicates region in F. (E) ceca sectioned and Wright stained. Scale bar, 1 mm. (D,E) Imaged via light microscopy. (F) A Vibrio-specific fluorescent probe, shown in orange with Cy3, revealed a strong signal within a darkly stained area of the intestine, near the ceca junction. Scale bar 100 μm. (G) Vibrio cells, shown in orange, observed inside of cells, at a specific junction between the midgut and the anterior digestive ceca. Scale bar 20 μm. Isopod nuclei are shown in blue, via DAPI. int., intestine.