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. 2023 Jan 11;13:1113237. doi: 10.3389/fmicb.2022.1113237

Figure 7.

Figure 7

The bony fish leech Pterobdella occidentalis (A) on the fins and body of the longjaw mudsucker (Gillichthys mirabilis), indicated by the arrow. Scale bar 1 cm. (B) Whole specimen, taken with a Pentax WG-III handheld camera. (C) Specimen embedded in Steedman’s wax and sectioned, imaged via light microscopy. Square indicates region in D. Scale bar, 1 mm. (D) Longitudinal section through the anterior end, showing general bacteria cells in a pair of mycetomes (shown in orange, via Eub338-Cy3), counterstained with DAPI, shown in blue. Square indicates region in E. Scale bar, 200 μm. (E) Close-up of bacterial cells, shown in orange, attached to the putative mycetome epithelia. Scale bar is 10 μm. (F) Transmission electron microscopy of a putative mycetome, revealing bacteria-like cells, some which appear to be dividing (arrows). Scale bar, 1 μm. (G) Longitudinal section through a near complete leech specimen, stained with DAPI. Scale bar, 1 mm. (H) Vibrio-specific cells within the crop (shown in orange, via Cy3), counterstained with DAPI, shown in blue. Scale bar, 200 μm. (I). Close-up of bacterial cells, shown in orange, within the crop. Scale bar, 10 μm. myc, mycetomes.