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. 2023 Jan 18;2023:8408574. doi: 10.1155/2023/8408574

Figure 1.

Figure 1

TRIM72 overexpression and knockdown in vivo and vitro. (a) Western blot analysis of tibial anterior muscle extracts using antibodies against TRIM72 after AAV-EV or AAV-TRIM72 transfection in mdx mice. AAV-TRIM72 was designed with 3xflag tag. (b) Representative immunofluorescence images of TRIM72 in tibial anterior muscle of mdx mice transfected with or without AAV. Scale bar, 100 μm. (c) Fluorescence indicated the successful transfection of LV-TRIM72 in C2C12 cells. Scale bar, 50 μm. (d) Western blot analysis of C2C12 cell extracts using antibodies against Flag after lentivirus transfection. LV-TRIM72 was designed with 3xflag tag. (e) The expression of TRIM72 mRNA in C2C12 cells with or without lentivirus transfection for 72 h. (f) C2C12 cells were transfected with si-TRIM72 for 72 h, and western blot was performed with antibodies against TRIM72. (g) The expression of TRIM72 mRNA in C2C12 cells with or without siRNA transfection for 72 h. Data were expressed as mean ± SEM. ∗p < 0.05, ∗∗p < 0.01, and ∗∗∗p < 0.001. AAV-EV: adeno-associated virus with empty vector; LV-EV: lentivirus with empty vector.